The establishment of ExT gel. (a) The schematic representation of synthesis of ExT hydrogel. (b) Possible hydrogen-bonding structures in the swollen ExT hydrogel. (c) The elastic modulus and expansion factor of expansion hydrogels with different AMPS-Na concentration (mean ± s.d., n = 3 samples each). (d) The elastic modulus and expansion factor of expansion hydrogels with different MBA concentration (mean ± s.d., n = 3 samples each). (e) The compression modulus of the expansion hydrogels with various expansion recipes (ExM [22,25], MAP [24], U-ExM [46,47], ExM-2 is defined as an expansion recipe which has the same molar concentration as the corresponding monomer component in ExT gel solution) (mean ± s.d., n = 3 samples each). (f) Measurements of surface evenness of ExT hydrogel after continuous sectioning with a vibratome. X is the direction of sectioning and Y is perpendicular to the direction of sectioning. The Root Mean Square (RMS) values in X direction and Y direction are (948.11 ± 35.62) nm and (953.51 ± 51.74) nm respectively (n = 3).