Figure 3.

Reclustering of Stele Cells Yields Distinct Subclusters of Vasculature Cell Types.

(A) Cells initially annotated as stele tissue were reclustered, resulting in six distinct subclusters cells, five of which contained >40 cells.

(B) Mean expression for previously identified cell-type–specific genes (Cartwright et al., 2009) in each cell is shown, allowing annotation of stele subcluster identities as shown in (A).

(C) Proportion of cells (circle size) and mean expression (circle color) of genes with cluster-specific and tissue-specific expression are shown, starting with known marker genes at the top, labeled with their common name (right) and their systematic name (left). Below, novel significant tissue-specific genes are shown with their systematic names, identified by principal graph tests (Moran’s I) as implemented in Monocle 3.

(D) Example expression overlays for cluster-specific genes identified by the principal graph test in (C).