Scheme 2. Examples of different methodologies for the labelling of the most reactive lysine. (a) STP used by Cravatt et al. for the investigation of global reactive lysines.29 PDB 3HY8 (b) Cellitti et al.'s fluorophenyl ester reagents, used for the labelling of K188 on the LC of Fab.32 (c) Sulfonyl acrylate modification of lysine on trastuzumab, by Bernardes et al.36.
