Scheme 6. Examples of affinity-guided approaches for site-selective lysine modification, using peptides. Red star = functionality. (a) Xiao and co-workers protocol, utilising FB protein encoded with 4-fluorophenyl carbamate and a fluorophore. The FB protein binds in the Fc region of IgG.58 PDB 1FC2. (b) The AJICAP platform for homogeneous lysine-conjugated ADC preparation, developed by Mendelsohn et al.61 A similar affinity peptide to Ig-BP, discovered by Ito et al., was used. PDB 6IQG.
