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. Author manuscript; available in PMC: 2021 Oct 14.
Published in final edited form as: J Vis Exp. 2020 Oct 1;(164):10.3791/61450. doi: 10.3791/61450

Figure 3. Immunohistochemistry stains.

Figure 3.

a. Schematic of cryosectioning brains 30um thick. Several sections in the middle are chosen for image analysis. b. Stroke only, immunohistochemistry (IHC) stains 15 days after stroke. Astrocytes (GFAP+) cells are shown in red, microglia (Iba1+) are green, and vessels (GLUT1+) are white. Stroke + Hydrogel condition, IHC 10d post injection, 15 days after stroke. Astrocytes (GFAP+) cells are shown in red, microglia (Iba1+) are green, hydrogel material is white.