Fig. 2.
Impact of different housing conditions in anxiety-like behaviour in the elevated plus-maze. Mean ± CI95. (A, B, C) % Permanence time, number of entrances and % entrances, respectively, in each arm in the elevated plus maze. The only effect noted was the effect of the arm, with no effect of housing and interaction; GEE with Bonferroni post hoc test, n = 10/group, # = effect of arm (p < 0.05). (D-E) Distance and latency to enter the open arm, respectively. Isolated mice showed higher latency to enter the open arm, suggesting that long-term isolation results in anxiety-like behaviour. Unpaired t-student test or Mann-Whitney (for non-parametric data), n = 9–10/group, * p < 0.05.