Fig. 4.

Immunoreactivity of ΔFosB in the hippocampus of mice housed in social vs isolated conditions. (A-B) Bregma reference used for immunohistochemistry [45]. Representative images of ΔFosB immunostaining in hippocampus of Social/Isolated mice and quantitative analyses for CA1 (C-E), CA2 (F-H), CA3 (I-K), and DG (L-N). In E, H, K, N, results are mean ± CI₉₅. Social isolation decreased ΔFosB, a neuronal activity marker, at DG. Unpaired t-student test or Mann-Whitney (for non-parametric data), n = 6/group, * p < 0.05. Thionine staining.