Fig. 5.

Immunoreactivity of BDNF in the hippocampus of mice housed in social vs isolated conditions. (A-B) Bregma reference used for immunohistochemistry [45]. Representative images of BDNF immunostaining in hippocampus of Social/Isolated mice and quantitative analyses for CA1 (C-E), CA2 (F-H), CA3 (I-K) and, DG (L-N). In E, H, K, N, results are mean ± CI₉₅. Social isolation decreased BDNF, a neurotrophic factor important for neuroplasticity, at CA3. Unpaired t-student test or Mann-Whitney (for non-parametric data), n = 5/group, * p < 0.05. Hematoxylin staining.