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. 2021 Oct 14;11(10):e587. doi: 10.1002/ctm2.587

FIGURE 1.

FIGURE 1

ATF4 is downregulated to promote chemoresistance in gastric cancer. (A) The gene expression profiles were analysed by Gene Set Enrichment Analysis (GSEA) in chemoresistant cells (SGC‐R and BGC‐R) and their parental sensitive cells (SGC7901 and BGC823), assessing the correlation to ATF4 target gene signatures in resistant cells compared to sensitive cells, ‘R’ represent drug‐resistant cells, ‘S’ represent sensitive cells. (B) Expression of ATF4 in chemoresistant cells (SGC‐R and BGC‐R) and their parental sensitive cells (SGC7901 and BGC823) was detected by western blotting, and Actin was used as loading control. (C) Cell viability of SGC‐R cells with ATF4 over‐expression, and DDP treatment for 24 h was measured by MTS assay. (D) The clonal growth of SGC‐R cells with ATF4 over‐expression, and DDP treatment for 7 days was assayed in Matrigel. (E) Cell viability of SGC7901 cells with ATF4 knockdown, and DDP treatment for 24 h was measured by MTS assay. (F) The clonal growth of SGC7901 cells with ATF4 knockdown, and DDP treatment for 7 days was assayed in Matrigel