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. 2021 Oct 14;11(10):e587. doi: 10.1002/ctm2.587

FIGURE 6.

FIGURE 6

Stabilisation of ATF4 reverses chemoresistance. (A) Cell viability of SGC‐R cells with DDP treatment, with/without D4476 (0, 25 or 50 μM) combination for 24 h was measured by MTS assay. (B) Apoptosis of SGC‐R cells with DDP treatment, with/without D4476 (25 μM) combination for 24 h was analysed by PI/Annexin V double staining. (C) Expression of apoptosis marker C‐PARP1 and ATF4 in SGC‐R cells with DDP treatment, with/without D4476 combination for 24 h was detected by western blotting. The short‐time and long‐time exposure results were shown as ATF4 (short) and ATF4 (long), respectively. Cell viability (D) and apoptosis (E) of SGC‐R cells with DDP treatment, with/without BTZ (1 μM) combination for 24 h was measured. (F) Expression of apoptosis marker C‐PARP1 and ATF4 in SGC‐R cells with DDP treatment, with/without BTZ (0.5 or 1 μM) combination for 24 h was detected by western blotting. The short‐time and long‐time exposure results were shown as ATF4 (short) and ATF4 (long), respectively. After ATF4 knockdown, cell viability of SGC‐R cells with DDP (5 μg/ml) treatment, with/without D4476 (25 μM) (G) or BTZ (1μM) (H) combination for 24 h was measured by MTS assay. (I) After ATF4 knockdown, expression of C‐PARP1 in SGC‐R cells with DDP (5 μg/ml) treatment, with/without D4476 (up) or BTZ (down) combination for 24 h was detected by western blotting