Figure 3. Hydrogen-rich saline inhibited SAH-induced autophagy activation in the hippocampus. (a) Expression of autophagy-related proteins, Beclin-1 and LC3 in the hippocampus of mice after SAH were determined by Western blotting. (b-c) Quantification of Beclin-1 and LC3 protein levels in the hippocampus to actin loading control, hydrogen-rich saline decreased Beclin-1 and LC3 expression after SAH in mice. (d) Immunocytochemistry shows that hydrogen-rich saline downregulated LC3 expression in the hippocampus. n=6; p<0.05; ANOVA; mean ± SEM.
SAH: subarachnoid hemorrhage; HS: hydrogen-rich saline; ANOVA: analysis of variance; SEM: scanning electron microscope.