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. 2021 Oct 8;36(8):e360804. doi: 10.1590/ACB360804

Figure 5. Rapamycin stimulates autophagy and reversed the neuroprotective effect of hydrogen-rich saline. (a) Hydrogen-rich saline increased the neurological score (n=6, p<0.05). (b) Hydrogen-rich saline alleviated brain water content significantly after SAH, while aggravated it after rapamycin administration (n=6, p<0.05). (c) Hydrogen-rich saline alleviated BBB permeability after SAH, while aggravated it after rapamycin administration (n=6, p<0.05). (d) TUNEL assay showed that autophagy activator increased neuronal death, and reversed the neuroprotective effect of hydrogen-rich saline. (e) Expression of autophagy-related proteins, LC3 and Beclin-1 after SAH were determined by Western blotting. (f) Rapamycin increased the expression levels of LC3 and Beclin-1 significantly than the SAH+HS group (n=6, p<0.05). n=6; p<0.05; ANOVA; mean ± SEM.

Figure 5

SAH: subarachnoid hemorrhage; HS: hydrogen-rich saline; Rap: rapamycin; BBB: blood-brain barrier; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; ANOVA: analysis of variance; SEM: scanning electron microscope.