Figure 2. ARG1-expressing myeloid cells accumulate in human breast cancer tissue.

Lung cancer (n = 10), melanoma (n = 10), and breast cancer (n = 60) tissue microarrays (TMAs) were used for immunofluorescence staining. (A) CD68⁺ myeloid cell number per mm² tissue area, and (B) percentage of ARG1⁺ cells in total CD68⁺ cells from lung cancer, melanoma, and breast cancer tissues. (C) CD68⁺ myeloid cell number per mm² tissue area, and (D) percentage of ARG1⁺ cells in total CD68⁺ cells from breast cancer (BC) subtypes. (E) ARG1⁺CD68⁺ myeloid cell number per mm² tissue area from breast cancer based on cancer stage. (F) Representative immunofluorescence images of stained paraffin-embedded human cancer tissue. Scale bars: 500 μm (whole-tissue images); 100 μm (enlarged images). Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, by 2-tailed, unpaired Student’s t test with Welch’s correction.