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. 2021 Oct 15;131(20):e129466. doi: 10.1172/JCI129466

Figure 6. A repertoire of potent neoantigen-specific TCRs.

Figure 6

Healthy donor T cells were electroporated with in vitro–transcribed TCR α and β chains. (A) Flow cytometric plots of A*01:01/ILDTAGKEEY-tetramer–stained cells. Negative controls were cells electroporated without mRNA (EN, electroporated nothing). (B and C) Electroporated T cells were coincubated overnight with peptide-pulsed IHW01161 cells. (B) IFN-γ ELISA with 10 μM peptide. ****P < 0.0001, by 1-way ANOVA with Tukey’s correction for multiple comparisons. Error bars represent the SEM of biological triplicates. (C) 4-1BB peptide titration assay. Plots show the mean with SD of biological triplicates. ****P < 0.0001, by 2-way ANOVA with Sidak’s correction for multiple comparisons.