Figure 3.

Generation of neural progenitors for transplantation and the neural markers of MGE neural progenitors during the process of differentiation. (a) Representative images were immunoreactive for neuroepithelium marker PAX6 and NSC marker Nestin on day 4. (b) Coimmunostaining SOX1 and PAX6 at day 10. (c) Differentiated hESCs expressed forebrain marker, FOXG1, but no spinal cord marker, HOXB4. (d) Coexpressing MGE neural progenitor marker NKX2.1 and FOXG1 on day 14. By 49 days in culture, neurons were positive for (e) GABA and (f) CHAT. Scale bars = 50 μm.