TABLE 1.
Type of comparison | No. of input copies | No. of HIV DNA copies/reaction in calculated standard replicate
|
Mean no. of HIV DNA copies/reaction | CV (%) | Maximum Z score | |||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | |||||
Intra-assay | 5 | 5 | 6 | 5 | 5 | 5 | 6 | 0.23 |
25 | 10 | 19 | 17 | 18 | 16 | 26 | 1.34 | |
100 | 97 | 122 | 44 | 89 | 88 | 37 | 2.07 | |
1,000 | 2,579 | 2,670 | 1,619 | 1,475 | 2,085 | 30 | 1.21 | |
10,000 | 10,000 | 7,782 | 4,943 | 7,661 | 7,596 | 27 | 1.44 | |
Interassay | 5 | 3 | 2 | 7 | 7 | 5 | 53 | 2.56 |
25 | 61 | 46 | 34 | 41 | 46 | 25 | 1.20 | |
100 | 118 | 71 | 80 | 70 | 85 | 27 | 1.07 | |
1,000 | 1,344 | 1,773 | 2,136 | 849 | 1,525 | 36 | 1.89 | |
10,000 | 7,112 | 6,878 | 10,000 | 7,507 | 7,874 | 18 | 0.77 |