Fig. 2.

FXR positively regulates expression of intestinal markers in gastric cells. (a) Left: Western blot for intestinal markers and miR-1 targets in GES-1 cells infected with a FXR expression vector. Right: Quantification of Western blot analysis results normalized to β-actin. (b) miR-1 expression in GES-1 cells detected by qRT-PCR at 48 h post-transfection. U6 was used as an internal control. (c) Left: AGS cells were transfected with siFXR and intestinal markers were examined by Western blotting. Right: quantification of Western blot analysis results normalized as in 2a. (d) qRT-PCR analysis of miR-1 expression in AGS cells transfected with a FXR-specific siRNA. *p < 0.05; **p < 0.01