Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 12;44(5):1119–1131. doi: 10.1007/s13402-021-00622-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — SNAI2 suppresses miR-1 transcription in BA-induced IM cells. (a, b) qRT-PCR analysis of SNAI2 and miR-1 expression in GES-1 and AGS cells with SNAI2 overexpression or knockdown. (c) miR-1 expression enhancement by ago-miR-1 (100 nM) in GES-1 cells. (d) Upregulation of intestinal markers and miR-1 targets induced by SNAI2 in GES-1 cells is abolished by miR-1 overexpression. (e) SNAI2 inhibits miR-1 promoter activity in GES-1 cells. (f, g) ChIP assay showing direct binding of SNAI2 to the miR-1 promoter in GES-1 cells. M: Marker. (h) qRT-PCR of ChIP products validating binding of SNAI2 to the miR-1 promoter. *p < 0.05; **p < 0.01. N.S., not significant