Figure 1.
Single-cell RNA-seq analysis of hPSC-AOs
(A) Uniform manifold approximation and projection (UMAP) plot illustrating five cell clusters in the hPSC-AOs. n = 1 biological replicate.
(B) UMAP and violin plots showing the expression of genes FOXJ1, MUC5B, KRT5, and MKI67.
(C) Enrichment analysis of hPSC-AOs using genes highly expressed in adult human ciliated or proximal ciliated cells.
(D) Correlation analysis of genes with cell fates in hPSC-AOs and adult human lung cells.
(E) Phase contrast image of a representative ciliated-like cell. Scale bar, 30 μm.
(F) UMAP and violin plots showing the expression of SARS-CoV-2 entry factors, including ACE2, CTSL, FURIN, TMPRSS2, and NRP1.
(G) Representative confocal images of hPSC-AOs co-stained with antibodies recognizing ACE2 and cilia marker acetyl-α-tubulin (AATB). 4′,6-diamidino-2-phenylindole (DAPI) stains nuclei. Scale bar, 20 μm.
(H) Representative confocal images of hPSC-AOs co-stained with antibodies recognizing ACE2 and ciliated cell marker FOXJ1. DAPI stains nuclei. Scale bar, 100 μm.