Figure 2.

hPSC-AOs are permissive to SARS-CoV-2 infection

(A) Relative SARS-CoV-2 RNA expression levels in hPSC-AOs at 48 hpi (MOI = 0.2). Total viral RNA from infected hPSC-AOs was analyzed by qRT-PCR for the presence of N transcripts relative to ACTB.

(B) Representative confocal images of hPSC-AOs at 48 hpi (MOI = 0.2) co-stained with antibodies recognizing SARS-CoV-2 nucleocapsid (SARS-N) protein and ciliated cell marker FOXJ1. Scale bar, 100 μm.

(C) Representative 3D confocal images of hPSC-AOs at 48 hpi (MOI = 0.2) co-stained with antibodies recognizing SARS-N and AATB. Scale bar, 10 μm.

(D) Read coverage on viral transcriptome in the mock- and SARS-CoV-2-infected hPSC-AOs (MOI = 0.2). Schematic shows the SARS-CoV-2 genome. Coverage is normalized per million reads.

(E and F) PCA (E) and sample clustering (F) on the mock- and SARS-CoV-2-infected hPSC-AOs.

(G) Volcano plot showing the gene expression changes between mock- and SARS-CoV-2-infected hPSC-AOs.

Data in (A) are presented as mean ± SEM (n = 3 biological replicates). The p values were calculated by unpaired two-tailed Student’s t test. ^∗∗∗∗p < 0.0001.

See also Video S2.