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. 2021 Oct 14;11:20491. doi: 10.1038/s41598-021-99781-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic analysis of wfs1a^−/− and wfs1b^−/− zebrafish. (A) Zebrafish were imaged at 30, 50 and 80 hpf. Scale bars represent 1 mm. (B–D) Zebrafish length at 30 hpf (B), 50 hpf (C) and 80 hpf (D). (E,F) Zebrafish head-trunk angles at 50 hpf (E) and 80 hpf (F). Zebrafish length and head tail angle was measured using ImageJ and data plots represent mean ± SEM (n = 10). Statistical significance was determined by One-Way ANOVA with Bonferroni multiple comparisons. **p < 0.01; ***p < 0.001; hpf hours post-fertilisation, SEM standard error of the mean.