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. 2021 Oct 14;11:20406. doi: 10.1038/s41598-021-00088-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The fecal microbiota composition of mice orally transplanted with patients with IBD/D⁻ or IBD/D⁺ and healthy volunteers (HVs). (A) Effects on OTU richness (a), Shannon’s index (b), principal coordinate analysis (PCoA) plot based on Jensen-Shannon analysis (c), and phylum level (d). (B) Effects on gut microbiota composition indicated by Cladogram between the feces of normal control mice (CON) and mice transplanted with HV (a), between the feces of HVs and IBD/D⁻ patients (b) and between the feces of HVs and IBD/D⁺ patients (c). (C) Effects on the levels of families Bacteroidaceae (a), Enterobacteriaceae (b), Enterococcaceae (c), Lactobacillaceae (d), and Prevotellaceae (e). The gut microbiota composition was analyzed by using using Illumina iSeq 100. (D) Effects on the fecal LPS levels. Data values were indicated as mean ± SD (NC n = 6; HV-F n = 4; IBD/D⁻ n = 8; IBD/D⁺ n = 7: each n value is the average obtained from 3 mice). Means with same letters are not significantly different (p < 0.05). (A)(a,b), (C)(a,b,d,e), and (D), Kruskal–Wallis test (nonparametric test); (B)(c), ANOVA with Tukey’s multiple comparisons test.