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. 2021 Oct 14;7:293. doi: 10.1038/s41420-021-00675-x

Fig. 3. Establishing the mETC-deficient cell models.

Fig. 3

AC Real-time PCR analysis of COX I, COX II, COX IV and CYTB mRNA (A) (N = 6) and Western blotting analysis of CYTB protein (B, C) (N = 3) in HCC cells after treatment with EB for 5 days. DF Real-time PCR analysis of NDUFS1 mRNA (D) (N = 6) and Western blotting analysis of NDUFS1 protein (E, F) (N = 3) in HCC cells after transfected with NDUFS1 lentivirus. Results are presented as mean ± SD, group differences were tested by Mann–Whitney U test.