FIG. 1.

Structure and expression of SLK. (A) Schematic representation of SLK and percent similarities (in parentheses) to other proteins. The catalytic domain (black) is most similar to LOK and MST1/2, two Ste20-related kinases. SLK is highly homologous to M-NAP (grey) and to AT1-46 (white) in the carboxy-terminal region. Numbers in brackets represent SLK amino acid (aa) residues. The Ste20 signature sequence (TPYWMAPE), caspase 3 consensus cleavage site (DTQD), and a putative SH3 binding domain (PPEPE) are shown. Numbers above the different domains denote the amino acid residues representing the boundaries. (B) Schematic representation of the plasmid expression vectors used in this study. The Ste20 kinase (black), M-NAP (grey), and ATH (white) domains are indicated. SLK amino acid residues are indicated at the N and C termini.