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. 2021 Oct 14;11:20438. doi: 10.1038/s41598-021-99736-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of DLEU1 overexpression on expression and histone modification at ISGs in OSCC cells. (a) Venn diagram showing genes downregulated by DLEU1 knockdown and/or upregulated by DLEU1 overexpression detected by microarray analysis of Ca9-22 cells. (b) Pathway analysis of selected genes from (a). (c) qRT-PCR analysis of representative ISGs in the indicated OSCC cells infected with an empty lentivirus vector (EV) or DLEU1 expression vector. (d, e) ChIP-qPCR analysis showing levels of H3K27ac (d) and H3K4me3 (e) at TSS regions of the indicated genes in HSC3 cells infected with the indicated lentivirus vectors. *P < 0.05.