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. 2021 Oct 14;12(10):945. doi: 10.1038/s41419-021-04208-3

Fig. 6. The coiled-coil domain and the RING domain of TRIM31 were critical for the suppressor role of TRIM31.

Fig. 6

The MCF7 and ZR-75-30 cells were transfected with Flag-TRIM31 plasmid or TRIM31 truncation mutant (TRIM31-ΔRING or TRIM31-ΔC–C) plasmids or Flag-TRIM31-C36A plasmid, and the expression of TRIM31 and its truncation mutants were detected by western blot (bd). The proliferation (b), colony formation (c), and migration (d) of breast cancer cells were further detected and analyzed, Bar length: 100 μm. Data are shown as the mean ± SD of at least three independent experiments, and the significant level was identified by *P < 0.05, **P < 0.01, and ***P < 0.001.