Table 2.
Summary of cross reactivity study.
| Microorganisms | Hit rate (#detected/#tested) | Result | ||||
|---|---|---|---|---|---|---|
| A well | B well | |||||
| ORF1ab | RNase P | N | RNase P | |||
| 1 | Human coronavirus 229E | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 2 | Human coronavirus OC43 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 3 | Human coronavirus HKU1 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 4 | Human coronavirus NL63 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 5 | SARS-coronavirus | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 6 | MERS-coronavirus | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 7 | Adenovirus type 1 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 8 | Adenovirus type 2 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 9 | Adenovirus type 3 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 10 | Human Metapneumovirus | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 11 | Parainfluenza virus 1 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 12 | Parainfluenza virus 2 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 13 | Parainfluenza virus 3 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 14 | Parainfluenza virus 4 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 15 | Influenza A (H3N2) | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 16 | Influenza A (H1N1) | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 17 | Influenza B | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 18 | Enterovirus | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 19 | Respiratory syncytial virus | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 20 | Rhinovirus 1 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 21 | Rhinovirus 14 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 22 | Rhinovirus 7 | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 23 | Chlamydia pneumoniae | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 24 | Haemophilus influenzae | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 25 | Legionella pneumophila | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 26 | Mycobacterium tuberculosis | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 27 | Streptococcus pneumoniae | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 28 | Streptococcus pyogenes | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 29 | Bordetella pertussis | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 30 | Mycoplasma pneumoniae | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 31 | Candida albicans | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 32 | Pseudomonas aeruginosa | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 33 | Staphylococcus epidermis | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 34 | Streptococcus salivarius | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 35 | Staphylococcus aureus | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
| 36 | Human nasal wash | 0% (0/3) | 100% (3/3) | 0% (0/3) | 100% (3/3) | Negative |
RNA extracts from all tested microorganisms as well as direct human nasal wash were confirmed to not cross-react with PNA RT-LAMP assay.