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. 2021 Oct 14;10(10):69. doi: 10.1038/s41389-021-00358-3

Fig. 1. GP73 promotes AFP secretion.

Fig. 1

A GP73 overexpression decreases the protein level of intracellular AFP and increases the level of extracellular AFP. The protein levels of GP73 and AFP in cell lysate and supernatant were measured by western blotting in HepG2 and PLC cells transfected with PCDNA3-GP73 or PCDNA3 (as a control). β-actin or GST was respectively used as an intracellular or extracellular loading control. The mRNA level of AFP in cell lysate was measured by Real-time PCR. B GP73 knockout increases the protein level of intracellular AFP and decreases the level of extracellular AFP. The protein levels of GP73 and AFP in cell lysate and supernatant were measured by western blotting in HepG2 and PLC cells mediated GP73 knockout by CRISPR/Cas9 system. β-actin or GST was respectively used as an intracellular or extracellular loading control. The mRNA level of AFP in cell lysate was measured by Real-time PCR. C GP73 overexpression increases the secretion level of AFP. Stable transfected HepG2 and PLC cells overexpressed GP73. Cell culture supernatant was collected and AFP level was measured by ELISA. D GP73 knockout decreases the secretion level of AFP. The expression of GP73 in HepG2 and PLC cells were knockout by CRISPR/Cas9 system. Cell culture supernatant was collected and AFP level was measured by ELISA. Error bars represent S.D. *p < 0.05, **p < 0.01.