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. 2021 Oct 14;10(10):69. doi: 10.1038/s41389-021-00358-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A, B GP73 interacts with AFP in vivo. A HEK293T cells co-transfected with FLAG-AFP and HA-GP73 were lysed with IP lysis buffer and subjected to immunoprecipitation with anti-HA or anti-FLAG antibodies followed by western blotting with anti-HA or anti-FLAG antibodies. B HepG2 cells were lysed with IP lysis buffer and subjected to immunoprecipitation with anti-AFP or anti-GP73 antibodies followed by western blotting with anti-AFP or anti-GP73 antibodies. C GP73 co-localizes with AFP in cytoplasm. HepG2 cells was stained with GP73 antibody (red) and AFP antibody (green). DAPI (blue), nucleus. Scale bar, 5 µm. D GP73 localizes in the Golgi apparatus. HepG2 cells was stained with GP73 antibody (red) and TGN46 (green). DAPI (blue), nucleus. Scale bar, 5 µm. E GP73 interacts with AFP in vitro. GST pull-down assay was performed by using His-GP73 and GST or GST-AFP proteins in vitro and analyzed by western blotting with anti-His antibody.