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. 2021 Oct 14;10(10):69. doi: 10.1038/s41389-021-00358-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A HLE is AFP and AFPR negative cell line. The protein levels of AFP and AFPR in HLE cells treated with or without GP73 overexpression supernatent (GP73-OE SP) were analyzed by western blotting. β-actin was used as a control. B, C GST-AFP promotes cell proliferation, migration, and invasion only in HepG2 cells. MTT assay (B) and Transwell assay (C) were conducted with HepG2 and HLE cells with or without GST-AFP treatment. Scale bar, 200 μm. D, E GST-GP73 promotes cell proliferation, migration, and invasion both in HepG2 and HLE cells. MTT assay (D) and Transwell assay (E) were conducted with HepG2 and HLE cells with or without GST-AFP treatment. Scale bar, 200 μm. F AFP knockdown decreases GP73-mediated AFP secretion. The protein levels of GP73 and AFP in cell lysate and supernatant were measured by western blotting in HepG2 and PLC cells stably transfected with GP73 with or without AFP knockdown plasmid. β-actin or GST was respectively used as an intracellular or extracellular control. G, H AFP knockdown decreases cell proliferation, migration, and invasion of GP73-mediated secretion only in HepG2 cells. MTT assay (G) and Transwell assay (H) were conducted with HepG2 and HLE cells with treatment of cell culture supernatant in panel F. Scale bar, 200 μm. Error bars represent S.D. *p < 0.05, **p < 0.01.