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. 2021 Oct 14;11:20469. doi: 10.1038/s41598-021-99837-1

Figure 2.

Figure 2

The effects of BNIP3 downregulation on the expression of Beclin-1, P62, Atg3, and Pink1. (A) Results from qRT-PCR and Western blot indicated that sh-BNIP3 transfection showed high-efficiency BNIP3 gene silencing (n = 3 per group). (B,D) Representative Western blot bands of BNIP3, P62, and Pink1 in HTR-8/SVneo cells after sh-RNA transfection. β-Actin was used as a control (n = 3 per group). (C,D) Representative western blot bands of Beclin-1 and ATG3 in HTR-8/SVneo cells after sh-RNA transfection (n = 3 per group). β-Actin was used as a control. ns: non-significant, *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Full-length blots are presented in Supplementary Figure 2.