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. 2021 Oct 14;11:20472. doi: 10.1038/s41598-021-98940-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of piRNA precursors from representative embryonic piRNA clusters. (a and b) Structure of the piRNA clusters (Chr 7 (1) and Chr 10) and positions of individual primer sets. The regions of the IAP element sequence in each cluster are described with red bars (from piRBase in UCSC Genome Browser mm10) (upper). Quantitative RT-PCR for the expression analysis of piRNA precursors transcribed from embryonic piRNA clusters using E16.5 wild type and MORC3-cKO embryonic testes (bottom). Data is normalized by β-actin and is shown as means and SD (Error bar) from more than triplicate PCR reactions. Significant differences (^*p < 0.05 by the t test) between wild type and MORC3-cKO data using primer sets for the Chr 7 (1)-1, 4, and 5 positions (a) and Chr 10–1 and 2 positions (b) are shown. (c) Schematic diagram of de novo DNA methylation pathway mediated piRNAs biogenesis involved with MORC3.

Expression of piRNA precursors from representative embryonic piRNA clusters. (a and b) Structure of the piRNA clusters (Chr 7 (1) and Chr 10) and positions of individual primer sets. The regions of the IAP element sequence in each cluster are described with red bars (from piRBase in UCSC Genome Browser mm10) (upper). Quantitative RT-PCR for the expression analysis of piRNA precursors transcribed from embryonic piRNA clusters using E16.5 wild type and MORC3-cKO embryonic testes (bottom). Data is normalized by β-actin and is shown as means and SD (Error bar) from more than triplicate PCR reactions. Significant differences (^*p < 0.05 by the t test) between wild type and MORC3-cKO data using primer sets for the Chr 7 (1)-1, 4, and 5 positions (a) and Chr 10–1 and 2 positions (b) are shown. (c) Schematic diagram of de novo DNA methylation pathway mediated piRNAs biogenesis involved with MORC3.