Fig. 2. Loss of NF-YA delays regenerative myogenesis in vivo.

a Representative immunostaining for eMyHC (red) and laminin (green) on TA muscle sections of NF-YA^fl/fl and NF-YA^cKO mice 5, 15 and 25 days after CTX injury. Nuclei were identified by DAPI staining. Scale bar: 100 µm. b Relative mRNA levels of myogenic markers Pax7, Myf5, Myogenin, eMyHC in whole 5-days injured TA muscles of NF-YA^fl/fl and NF-YA^cKO mice measured by RT-qPCR. Transcript levels of NF-YA^fl/fl and NF-YA^cKO uninjured mice have been arbitrarily set at 1. Data represent mean ± s.d. (one-Way Anova: Pax7 F(3,8)=9.66, p = 0.0049; Myf5 F(3,8)=44.63, p < 0.0001; MyoG F(3,8)=9,99, p = 0.0044; eMyHC F(3,8)=170.8, p < 0.0001; n = 3 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs control; ^##p < 0.01, ^####p < 0.0001 vs NF-YA^fl/fl). c Western blot analysis performed on whole protein extracts of uninjured (control) and 5-days injured TA muscles of NF-YA^fl/fl and NF-YA^cKO mice. Immunoblots represent protein levels of Pax7, eMyHC, Myogenin and Tubulin. n = 3 experiments. d Western blot analysis of eMyHC on whole protein extracts of 15-days injured TA muscles of NF-YA^fl/fl and NF-YA^cKO. Tubulin was used as loading control. n = 3 experiments.