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. 2021 Oct 14;12(10):948. doi: 10.1038/s41419-021-04253-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A RIP was performed using an antibody against AGO2 on extracts from monocytes and HCAECs. B CircNPHP4 was performed using a circNPHP4-specific probe and control probe in HCAECs. The enrichment of circNPHP4 and microRNAs were detected by qRT-PCR and normalized to the control probe. C Co-localization between circNPHP4 and miR-1231 was observed by RNA in situ hybridization in HCAECs. Nuclei were stained with DAPI. Scale bar = 20 µm. D Schematic showing the predicted miR-1231 sites in circNPHP4. A Luciferase assay where monocytes were co-transfected with a scrambled control, miR-1231 mimic, and a luciferase reporter plasmid containing wild-type circNPHP4 (circNPHP4-WT) (E) or mutant circNPHP4 (circNPHP4-mut) (F). G qRT-PCR showed the level of circNPHP4 in the streptavidin-captured fractions from the monocytes and HCAECs lysates after transfection with biotinylated miR-1231 or control RNA. H Expression of miR-1231 was analyzed using qRT-PCR following circNPHP4 knockdown. Data are presented as means ± SD; a significant difference was identified with Student’s t test. *P < 0.05; **P < 0.01; ns (not significant).