Table 2.

Biophysical properties of K_v7.2 homomers in CHO hm1 cells co-transfected with GFP and PIP5K.

Transfection	n	Leak subtracted I at +20 mV (pA)	G/GmaxV1/2(mV)	G/Gmaxk(mV/e fold)
GFP	12	27.3 ± 4.0*	−65.8 ± 9.2*	0.7 ± 0.2*
GFP + WT	31	567.7 ± 43.3	−30.5 ± 0.5	5.9 ± 0.7
GFP + R214Q	16	299.6 ± 35.5*	−31.4 ± 0.5	4.3 ± 0.4*
GFP + K219N	12	150.4 ± 32.4*	−13.7 ± 1.7*	6.5 ± 0.7
GFP + R325Q	11	63.9 ± 7.5*	−65.1 ± 3.6*	1.2 ± 0.4*
GFP + R353Q	13	310.2 ± 39.9*	−31.7 ± 1.1	5.0 ± 1.1
GFP + R214Q/K219N	15	284.1 ± 19.5*	−9.7 ± 0.6*	5.6 ± 0.4*
GFP + R214Q/K219N/R353Q	14	312.4 ± 38.5*	−9.8 ± 1.0*	5.5 ± 0.6
GFP + WT + PIP5K	33	2164.0 ± 92.4^	−40.3 ± 0.6^	2.3 ± 0.3^
GFP + R214Q + PIP5K	15	1500.8 ± 138.3^†	−38.7 ± 0.7^	2.2 ± 0.4^
GFP + K219N + PIP5K	12	981.9 ± 92.9^†	−29.4 ± 0.8^†	3.2 ± 0.6^
GFP + R325Q + PIP5K	11	75.0 ± 9.2†	−33.3 ± 3.0^†	8.5 ± 1.4^†
GFP + R353Q + PIP5K	16	1373.5 ± 79.5^†	−42.4 ± 1.2^	1.2 ± 0.3^
GFP + R214Q/K219N + PIP5K	19	997.7 ± 70.4^†	−20.5 ± 0.9^†	3.8 ± 0.6^†
GFP + R214Q/K219N/R353Q + PIP5K	14	492.2 ± 48.4†	−20.8 ± 1.2^†	4.3 ± 0.9^

n number, NA not applicable, Leak subtracted peak current (I) measured at +20 mV, V_1/2 half-activation potential, k the slope factor, τ activation time constant measured at +20 mV. All values are calculated from leak subtracted current. V_1/2 and k are calculated from normalized conductance G/G_max. Mean ± SEM (GFP + selected variant: *p < 0.05 for K_v7.2 WT vs. mutant; ^†p < 0.05 for K_v7.2 WT + PIP5K vs. mutants + PIP5K; ^p < 0.05 for the difference between -PIP5K and +PIP5K within the same transfection. The source data for Table 2 is available in Figshare⁹³.