. 2021 Oct 1;8:734334. doi: 10.3389/fnut.2021.734334

Table 4.

Dietary I3C and DIM alteration of FMO1 in rat liver microsomes and FMO/CYP-mediated metabolism of N,N-dimethylaniline, nicotine and tamoxifen (145, 153).

Diet^a	FMO1 Protein (% Control)^b	UL-ring ¹⁴C-N,N-Dimethylaniline (FMO/CYP)^c	(S)-5-³H-Nicotine (FMO/CYP)^d	³H-N-methyl-Tamoxifen (FMO/CYP)^e
Control	100	1.11	1.43	0.79
1,000 ppm I3C	93	0.22	1.11	0.53
2,500 ppm I3C	10	0.07	0	0.20
1,000 ppm DIM	13	0.14	0	0.32
2,500 ppm DIM	3	0.02	0.08	0.31

Male Fischer 344 rats were fed AIN-76A diet containing I3C or DIM for 4 weeks.

FMO1 protein levels in liver microsomes measured by western blotting using polyclonal antibody to pig FMO1.

[¹⁴C]-Dimethylaniline-N-oxide (FMO) and methylaniline (CYP) measured by HPLC with on-line radiochemical detection.

(S)-[³H]-Nicotine-N-1'-oxide (FMO), nornicotine (CYP) and nicotine-Δ^1,5-iminium ion (CYP) measured by HPLC with on-line radiochemical detection.

[³H]-Tamoxifen-N-oxide (FMO), N-desmethyl-tamoxifen (CYP) and 4-hydroxy-tamoxifen (CYP) were determined by TLC and radioscanning using a System 2,000 imaging scanner (Bioscan, Inc., Washington, DC).