FIGURE 6.

Effect of storage time, storage temperature and DNAse treatment on RNA or DNA yield from uEV. Total RNA yield (expressed as pg/ml of urine) was quantified using pico 6000 RNA chip in Agilent 2100 Bioanalyzer. DNA content was quantified using Qubit dsDNA HS Assay kit. a. RNA yield and urine storage time. uEV were isolated from urines stored for up to 4 years at ‐80°C including samples from healthy controls and donors with T1D or T2D and normo‐ micro‐ or macroalbuminuria. Differences in the RNA yield between time points were tested using One‐way ANOVA and Bonferroni Post‐Hoc test. b. RNA yield and urine storage temperature (‐20°C, ‐80°C). uEV were isolated from healthy controls and donors with T1D and normo‐ or macroalbuminuria including urines stored for up to 4 years. T‐test for paired samples was used to assess the difference between groups. c and d. DNAse treatment. RNA yield (C) and DNA concentration (D) of uEV‐RNA treated with and without DNAse during RNA isolation. uEV were isolated from healthy control samples. T‐test for paired samples was used to assess difference between groups. Months (mo), type 1 diabetes (T1D), type 2 diabetes (T2D), urinary extracellular vesicles (uEV), with DNAse treatment (+DNAse), without DNAse treatment (‐DNAse)