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. 2021 Oct 14;10(12):e12158. doi: 10.1002/jev2.12158

FIGURE 7.

FIGURE 7

Effect of urine storage temperature (‐20°C versus ‐80°C) on mRNA sequencing of uEV. Results depict 24 uEV‐mRNA samples including 4 pairs of urine samples stored in ‐20°C versus ‐80°C (pairs 1–4) and 16 more samples stored at –80°C. All samples were stored for 1 year at the indicated temperatures. Data was TMM normalized and analysed using edgeR. a. Alignment of raw sequencing reads to human genome using STAR. b. Sample to sample correlation heatmap (10136 genes, with > 1 raw count in at least 90% of the samples). c. Principal component analysis (10136 genes, with > 1 raw count in at least 90% of the samples). d. Heatmap of 22 kidney‐enriched genes (www.proteinatlas.org). e. Differential expression analysis of the ‐20°C versus ‐80°C pairs (4 pairs) (12642 genes, with > 5 raw counts in at least 50% of the samples). Volcano plot depicts differentially expressed genes. Adjusted p‐values lower than 0.05 and log2 fold change ≥ 0.75 or ≤ ‐0.75 are highlighted in red. Principal component analysis (PCA), urinary extracellular vesicle (uEV)