FIGURE 8.

Gene length, GC content and overlap with P‐body transcripts of up‐ and down‐regulated genes in uEV isolated from urines stored at ‐20°C versus ‐80°C. a. Transcript length (if several transcripts were retrieved, the average length was calculated). b. Gene %GC content. c. Distribution of gene %GC content in ‐20°C and ‐80°C samples. Genes with raw counts ≥ 5 were selected for the analysis. T‐test for four paired samples (Type 1 Diabetes) was used to assess differences between groups per percentage category. Urinary extracellular vesicles (uEV). d. Venn diagram of P‐body enriched transcripts and transcripts upregulated or downregulated in uEV by urine storage at ‐20°C versus ‐80°C