FIGURE 11.

Comparison of miRNAs enriched in the uEV isolates from this study or in previous publications evaluating uEV isolation methods. The list of miRNA enriched in uEV isolates was build based on healthy male ‐derived data from two different miRNA platforms and Vesiclepedia. The plots show the miRNA expression levels from different miRNA‐seq studies including only men. a. Barreiro et al., 2020. uEV were isolated from healthy controls and individuals with type 1 diabetes and macroalbuminuria. Raw counts were TMM normalized using edgeR. b. Mussack et al., 2019. uEV were isolated from healthy controls. Trimmed sequences were aligned using Chimira and counts were TMM normalized using edgeR. c. Srinivasan et al., 2019. Data selected for our analysis is derived from uEV or exRNA isolated from pooled urines of healthy controls (technical replicates). RPMSmiR counts were log2 transformed. d. uEV isolated by UC from urine sample pairs (4 pairs) stored at ‐20°C and ‐80°C. Urine samples included were from individuals with type 1 diabetes and micro‐ or macroalbuminuria. Hsa‐miR‐10a‐5p was found to be upregulated in ‐20°C group (figure 9). Counts per million (CPM), exosome Isolation Kit Pan, human (Exosome isolation kit), false discovery rate (FDR), hydrostatic filtration dialysis (HFD), ultracentrifugation (UC), reads per million scaled miRNA (RPMSmiR), urinary extracellular vesicles (uEV), urine Exosome Purification and RNA Isolation Midi Kit (NG)