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. 2021 Oct 14;10(12):e12158. doi: 10.1002/jev2.12158

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© 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Summary of results and recommendations. Never frozen refers to uEV isolated from fresh urine and kept at +4°C for a maximum of 1 day. Electron microscopy (EM), not applicable (NA), not determined (ND), not compared but data presented in the manuscript supports the statement (No difference)*, overnight (ON), principal component analysis (PCA), sequencing (seq), urinary extracellular vesicles (uEV), with DNAse treatment (+DNAse), with pre‐clearing before freezing (+pre‐clearing), with protease inhibitor (+PI), without DNAse treatment (‐DNAse), without pre‐clearing before freezing (‐pre‐clearing), without protease inhibitor (‐PI).