TABLE 2.
Particle concentration and size distribution of uEV stored (‐80°C) up to 90 days in PBS or urine
| uEV storage modality, time | Mean concentration (particles/ml) | Mean diameter (nm) | Modal diameter (nm) | Median diameter (nm) | 10th percentile ‐ 90th percentile (nm) |
|---|---|---|---|---|---|
| Fresh | 4,8E12 ± 1,1E12 | 163,9 ± 7,4 | 124,3 ± 24,3 | 143,2 ± 18,6 | 82,1 ± 6,9 ‐ 254,5 ± 14,3 |
| PBS, d1 | 3,2E12 ± 2,9E11 | 170,6 ± 6,3 | 127,4 ± 40,7 | 163,6 ± 20,1 | 87,0 ± 6,6 ‐ 262,5 ± 15,7 |
| PBS, d14 | 1,4E12 ± 7,3E11 ** | 140,5 ± 11,1 | 117,4 ± 21,6 | 124,5 ± 9,0 | 73,3 ± 8,0 ‐ 213,0 ± 31,1 |
| Urine d14 | 2,0E12 ± 1,6E12 * | 124,5 ± 28,7 | 105,6 ± 14,7 | 104,8 ± 17,9 | 70,4 ± 13,1 ‐ 187,3 ± 63,9 |
| PBS, d90 | 3,4E12 ± 1,9E11 | 158,7 ± 9,3 | 111,1 ± 2,8 | 129,9 ± 8,1 | 86,4 ± 2,9 ‐ 254,4 ± 11,1 |
| Urine, d90 | 2,2E12 ± 1,4E11 | 168,6 ± 7,7 | 113,3 ± 5,9 | 141,5 ± 10,3 | 90,8 ± 4,6 ‐ 271,5 ± 9,9 |
| uEV stored in PBS | 3,20E12 ± 1,38E12 | 158,4 ± 13,8 | 120,1 ± 23,2 | 140,3 ± 20,3 | 82,2 ± 7,9 ‐ 246,1 ± 26,3 |
| uEV stored in urine | 3,01E12 ± 1,67E12 | 152,3 ± 26,0 | 114,4 ± 16,6 | 129,9 ± 23,4 | 81,1 ± 11,8 ‐ 237,8 ± 50,8 |
Values represent mean ± SD, n = 3 technical replicates, bolded values represent mean values of given storage modality across all time points ± SD, n = 12 for PBS stored uEV, n = 9 for uEV stored in urine. * = P < 0.05, ** = P < 0.01 compared to freshly isolated uEV using Bonferroni comparison of means. Days (d), phosphate buffered saline (PBS), urinary extracellular vesicles (uEV).