Figure 3.

In vivo transfection of FTS-PEI

(A) GFP expression after 48 h in tumor tissues and LNs. (B) Immunofluorescence staining of GFP in tumors and LNs. (C) Luciferase expression after 48 h in tumors and LNs. Data are presented as mean ± SEM, n = 3. p values were generated by two-tailed Student’s t test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (D) Fluorescence-activated cell sorting (FACS) analyses of GFP expression in fluorescence-labeled tumor cells (tdTomato⁺) in tumor tissues and DCs (CD11b⁺, MHC class II⁺) in LNs. (E) Comparison of pGFP transfection efficiency in tumors and LNs by PEI, OA-PEI, LA-PEI, and FTS-PEI, respectively.