Figure 3.

circHPS5 promotes HCC cell proliferation and migration

(A) shRNA against circHPS5 was designed to silence circHPS5 (sh-circHPS5) in HCC cells. Left: The expression of circHPS5 was evaluated using qRT–PCR; Right: The expression of HPS5 mRNA was evaluated using qRT-PCR. (B) The growth curves of cells were constructed using CCK-8 assays after transfection with sh-circHPS5 or sh-NC. (C) A colony formation assay was performed to evaluate cell proliferation. (D) EdU assays of HCC cells transfected with sh-circHPS5 or sh-NC were performed to evaluate cell proliferation. Scale bar,50 μm. (E) Flow cytometry was used to assess cell apoptosis. (F) Transwell experiments were used to assess cell invasion. Scale bar,50 μm. (G) The motility of cells transfected with sh-circHPS5 or sh-NC was examined using wound healing assays. Scale bar,100 μm. ∗∗p < 0.01, ∗∗∗p < 0.001.