Figure 6.

YTHDC1 promotes cytoplasmic export of m6A-modified circHPS5

(A) Immunoblot analysis of YTHDC1 after a pulldown assay showing its specific association with circHPS5. (B) RIP assays showing the association of YTHDC1 with circHPS5. IgG antibody served as a control. (C) YTHDC1 interacted with circHPS5 at the GGACU motif of circHPS5. (D) Cytoplasmic and nuclear mRNA fractionation experiments showing the knockdown of YTHDC1 (WT/mutant) and the expression of circHPS5 in different locations. (E) RNA-FISH showing that the increased nuclear staining of circHPS5 caused by si-YTHDC1 was rescued by overexpression of WT but not mutant YTHDC1. Scale bar,10um. ∗∗p < 0.01, ∗∗∗p < 0.001.