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. 2021 Sep 14;26:637–648. doi: 10.1016/j.omtn.2021.09.001

Figure 7.

Figure 7

circHPS5 promotes HCC development by sponging miR-370

(A) Two databases (TargetScan and RegRNA) were analyzed, and three miRNAs (miR-1183, miR-1299, and miR-370) that may have binding sites with circHPS5 were identified in the intersection. (B) Expression of the three miRNAs in HCC cells. (C) A pulldown assay further confirmed the binding of circHPS5 and miR-370 in HCC cell lines. (D) Growth curves of cells were constructed using CCK-8 assays after cotransfection with sh-circHPS5 and miR-370 inhibitor. (E) A colony formation assay was performed to evaluate cell proliferation. (F) EdU assays of HCC cells cotransfected with sh-circHPS5 and miR-370 inhibitor were performed to evaluate cell proliferation. Scale bar, 50um. (G) Flow cytometry was used to assess cell apoptosis. (H) Transwell experiments were used to assess cell invasion. Scale bar,50um. (I) Wound healing assay. Scale bar, 100um. ∗∗p < 0.01, ∗∗∗p < 0.001.