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. 2021 Sep 14;26:637–648. doi: 10.1016/j.omtn.2021.09.001

Figure 8.

Figure 8

circHPS5 promotes HCC progression by sponging miR-370 to regulate HMGA2

(A) The expression of HMGA2 in HCC tissues and normal tissues via TCGA database prediction. (B) The association between HMGA2 expression and TNM stage. (C) Kaplan–Meier survival curve showing the relationship between HMGA2 and overall survival time. (D–G) circHPS5 possesses a complementary sequence to the miR-370 seed region according to bioinformatics analysis. Relative luciferase activities were analyzed in 293T cells cotransfected with miR-370 inhibitor and the luciferase reporter vectors pGL3-circHPS5-WT or pGL3-circHPS5-Mut. Relative luciferase activities were analyzed in 293T cells cotransfected with miR-370 inhibitor and the luciferase reporter vectors pGL3-HMGA2-WT or pGL3-HMGA2-Mut. In addition, relative luciferase activities were analyzed in 293T cells cotransfected with miR-370 inhibitor or sh-circHPS5 and the luciferase reporter vectors pGL3-HMGA2-WT or pGL3-HMGA2-Mut. (H) FISH assay showing the location of circHPS5 and miR-370. Scale bar, 10um. (I) The relative expression of HMGA2 mRNA in cells transfected with sh-circHPS5 and miR-370 inhibitor was evaluated using qRT-qPCR. (J and K) The relative expression of HMGA2 protein in cells transfected with sh-circHPS5 and/or miR-370 inhibitor was evaluated using western blotting. ∗∗p < 0.01.