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. 2021 Oct 1;8:697236. doi: 10.3389/fcvm.2021.697236

Figure 5.

Figure 5

Effect of miR-491-5p and MMP-9 on ox-LDL-induced THP-1 macrophages. (A) The transfection efficiency of miR-491-5P mimic was determined using qRT-PCR. (B,C) MMP-9 mRNA and protein levels were detected in THP-1 macrophages transduced with miR-491-5P and miR-491-5P mimic+MMP-9-plasmid. (D–J) Transfected THP-1 macrophages were stimulated with 50 μg/mL ox-LDL. (D) Cell activity after mir-491-5P mimic transfection was assessed using CCK-8. (E,F) MMP-9 mRNA and protein were detected in ox-LDL-induced THP-1 macrophages transduced with mir-491-5p mimic and mir-491-5p mimic +MMP-9-plasmid. (G–I) ROS and MDA levels and SOD activity were examined using commercial kits. (J) Levels of inflammatory factors (TNF-α, IL-1β, and IL-6) were measured with ELISA. Data are presented as the mean ± SD, n = 6. Compared with the control group, **P < 0.01; compared with the ox-LDL+mimic-NC group, ##p < 0.01; compared with the ox-LDL + miR-491-5Pmimic+plasmid-NC group, &&P < 0.01.