Figure 3.

Impact of BTL-1 on inflammatory cytokines production in LPS-induced IPEC-J2 cell. The outcome of BTL-1 on IPEC-J2 cell viability (A,B). The consequences of BTL-1 on the expression of cytokines were determined by ELISA (C–F) and qPCR (G–J). The consequences of BTL-1 on the expression of cytokines were determined by ELISA. The cells were treated with BTL-1 (10, 20, and 50 μM) for 24 h and then treated with LPS (50 μg/mL) for another 1 h. The data shown are representative of three independent experiments. The results were expressed as the means ± SEM. ^#P < 0.05, ^##P < 0.01, compared to non-treated group, ^*P < 0.05, ^**P < 0.01 compared to LPS group.