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Inhibitory effects of BTL-1 on TLR4/NF-κB signaling pathway. BTL-1 inhibits TLR4/NF-κB signaling pathway as determined by qPCR (A–C) and western blotting (D–G). BTL-1 inhibits the translocation of phosphorylated P65 as determined by western blotting (D,H) and IF (I). The data are representative of three independent experiments. The results were expressed as the means ± SEM. #P < 0.05, ##P < 0.01, compared to non-treated group, *P < 0.05, **P < 0.01 compared to LPS group.
